目錄
編寫說明
第一章 分析化學(xué)實(shí)驗(yàn)基本要求 (1)
1.1 實(shí)驗(yàn)室安全常識(shí) (1)
1.1.1 實(shí)驗(yàn)室安全規(guī)則 (1)
1.1.2 實(shí)驗(yàn)室安全標(biāo)志 (2)
1.2 實(shí)驗(yàn)記錄與實(shí)驗(yàn)報(bào)告 (2)
1.2.1 實(shí)驗(yàn)記錄和實(shí)驗(yàn)報(bào)告注意事項(xiàng) (2)
1.2.2 實(shí)驗(yàn)報(bào)告內(nèi)容 (2)
1.3 化學(xué)試劑的一般知識(shí) (3)
1.3.1 化學(xué)試劑的規(guī)格 (3)
1.3.2 分析用純水 (3)
1.4 常用辭典、手冊(cè)及網(wǎng)上信息查詢簡介 (4)
1.4.1 辭典、手冊(cè) (4)
1.4.2 網(wǎng)上信息查詢 (4)
第二章 分析化學(xué)實(shí)驗(yàn)基本操 (5)
2.1 稱量 (5)
2.1.1 分析天平 (5)
2.1.2 稱量方法 (5)
2.2 玻璃儀器 (5)
2.2.1 放璃儀器的洗滌與干燥 (6)
2.2.2 玻璃儀器的使用 (7)
第三章 化學(xué)分析實(shí)驗(yàn) (10)
實(shí)驗(yàn)3.1 氯化鎖干燥失重的測(cè)定 (10)
實(shí)驗(yàn)3.2 生藥灰分的測(cè)定 (11)
實(shí)驗(yàn)3.3 氫氧化錦標(biāo)準(zhǔn)溶液的配制和標(biāo)定 (12)
實(shí)驗(yàn)3.4 食用醋分析 (14)
實(shí)驗(yàn)3.5 草酸含量測(cè)定 (15)
實(shí)驗(yàn)3.6 鹽酸標(biāo)準(zhǔn)溶液的配制與標(biāo)定 (17)
實(shí)驗(yàn)3.7 碳酸鹽和碳酸氫鹽的含量測(cè)定 (18)
實(shí)驗(yàn)3.8 混合堿的含量測(cè)定 (19)
實(shí)驗(yàn)3.9 高氯酸標(biāo)準(zhǔn)溶液的配制與標(biāo)定 (21)
實(shí)驗(yàn)3.10 乳酸鋪?zhàn)⑸湟褐械娜樗徜伜�量測(cè)定 (22)
實(shí)驗(yàn)3.11 EDTA標(biāo)準(zhǔn)溶液的配制與標(biāo)定（24）
實(shí)驗(yàn)3.12 水的硬度測(cè)定（25）
實(shí)驗(yàn)3.13 明磯中硫酸鋁餌的含量測(cè)定 (27)
實(shí)驗(yàn)3.14 硫代硫酸鈾標(biāo)準(zhǔn)溶液的配制與標(biāo)定 (29)
實(shí)驗(yàn)3.15 腆標(biāo)準(zhǔn)榕液的配制與標(biāo)定 (31)
實(shí)驗(yàn)3.16 腆量法測(cè)定維生素C的含量 (32)
實(shí)驗(yàn)3.17 高鍾酸餌標(biāo)準(zhǔn)榕液的配制與標(biāo)定 (34)
實(shí)驗(yàn)3.18 市售過氧化氫溶液的含量測(cè)定 (35)
實(shí)驗(yàn)3.19 硝酸銀標(biāo)準(zhǔn)溶液和硫氨酸鍍標(biāo)準(zhǔn)溶液的配制和標(biāo)定 (37)
實(shí)驗(yàn)3.20 法楊斯法測(cè)定氯化物 (39)
第四章 儀器分析實(shí)驗(yàn) (41)
實(shí)驗(yàn)4.1 阿司匹林片劑中乙酷水楊酸的含量測(cè)定 (41)
實(shí)驗(yàn)4.2 永停滴定法測(cè)定磺膠喃睫的含量 (43)
實(shí)驗(yàn)4.3 柱色譜法測(cè)定氧化鋁活度 (44)
實(shí)驗(yàn)4.4 柱色譜法分離菠菜中的植物色素 (4的
實(shí)驗(yàn)4.5 黃連藥材的薄層色譜鑒別 (48)
實(shí)驗(yàn)4.6 鄰硝基苯膠和對(duì)硝基苯膠的薄層分離 (49)
實(shí)驗(yàn)4.7 氨基酸的紙包譜鑒別 (50)
實(shí)驗(yàn)4.8 氣相色譜法測(cè)定町劑中乙醇的含量 (52)
實(shí)驗(yàn)4.9 高效液相色譜柱的性能考察及分離度測(cè)試 (53)
實(shí)驗(yàn)4.10 高效液相色譜法測(cè)定槐米中蘆丁的含量 (55)
實(shí)驗(yàn)4.11 維生素B2注射液的定性鑒別及定量分析 (57)
實(shí)驗(yàn)4.12 熒光分光光度法測(cè)定維生素B2片中核黃素的含量 (58)
實(shí)驗(yàn)4.13 感冒沖劑中銅含量的測(cè)定 (60)
實(shí)驗(yàn)4.14 紅外分光光度法測(cè)定苯甲酸和苯甲醇的結(jié)構(gòu) (61)
實(shí)驗(yàn)4.15 核磁共振波譜法測(cè)定水楊酸甲醋的結(jié)構(gòu) (63)
實(shí)驗(yàn)4.16 氣相色譜質(zhì)譜聯(lián)用技術(shù)對(duì)混合物中甲苯、氯苯和澳苯定性分析 (65)
實(shí)驗(yàn)4.17 液相色譜質(zhì)譜聯(lián)用技術(shù)對(duì)中藥黃苓中黃苓昔的定性和定量分析 (66)
第五章 設(shè)計(jì)性實(shí)驗(yàn)和綜合性實(shí)驗(yàn) (69)
實(shí)驗(yàn)5.1 膽磯中硫酸銅的含量測(cè)定(設(shè)計(jì)性實(shí)驗(yàn)) (69)
實(shí)驗(yàn)5.2 中藥牲丹皮中丹皮酣的含量測(cè)定(設(shè)計(jì)性實(shí)驗(yàn)) (70)
實(shí)驗(yàn)5.3 大黃中藍(lán)眼類化合物的柱色譜分離及組分的薄層鑒定(綜合性實(shí)驗(yàn)) (70)
附錄 (72)
附錄一 相對(duì)原子質(zhì)量表(1995年IUPAC) (72)
附錄三 常用緩沖溶液 (73)
附錄三 常用酸堿指示劑 (73)
附錄四 常用酸堿的理度、含量和濃度 (74)
Table of Contents
Chapter 1 Laboratory General Knowledge (75)
1.1 Laboratory Safety (75)
1.1.1 Safety Rules (75)
1.1.2 Safety Signs (76)
1.2 The Laboratory Notebook (76)
1.2.1 Experiment Results Recording Policies (76)
1.2.2 Contents of a Laboratory Notebook (77)
1.3 Reagents (77)
1.3.1 Grades of Chemicals (77)
1.3.2 Grades of Purified Water (78)
1.4 Commonly Used DictionariestManuals and on-line Informations (78)
1.4.1 Dictionaries and Manuals (78)
1.4.2 On-line Informations (78)
Chapter 2 Experimental Techniques (79)
2.1 Weighing (79)
2.1.1 Analytical Balance (79)
2.1.2 Weighing Methods (79)
2.2 Glassware (80)
2.2.1 Cleaning and Drying Glassware (81)
2.2.2 Use of Volumetric Glassware (81)
Chapter 3 Chemical Analysis (86)
Experiment 3.1 Determination of Drying Loss in Barium Chloride (86)
Experiment 3.2 Determination of the Ash Content in the Crude Drug (87)
Experiment 3.3 Preparation and Standardization of Sodium Hydroxide Solution (88)
Experiment 3.4 Titration of a Commercial Vinegar Product (90)
Experiment 3.5 Analysis of the Oxalic Acid (91)
Experiment 3.6 Preparation and Standardization of Hydrochloride Acid Solution (93)
Experiment 3.7 Analysis of a Mixture of Carbonate and Bicarbonate (94)
Experiment 3.8 Titration of a Mixed &se (96)
Experiment 3.9 Preparation and Standardization of Perchloride Acid Solution (97)
Experiment 3.10 Determination of the Sodium Lactate in Sodium Lactate Injection (99)
Experiment 3.11 Preparation and Standardization of EDTA Solution (100)
Experiment 3.12 Determination of the Water Hardness (102)
Experiment 3.13 IRtermination of the Aluminum Potassium Su1fate in Alum (104)
Experiment 3.14 prepration and Standardization of Sodium Thiosulfate Solution (105)
Experiment 3.15 Preparation and Standardization of Iodine Solution (107)
Experiment 3.16 Iodimetric Titration of Vitamin C (109)
Experiment 3.17 Preparation and Standardization of Potassium Permanganate Solution (111)
Experiment 3.18 Determination of the Commercial Hydrogen Peroxide Solution (112)
Experiment 3.19 Preparation and Standardization of the Silver Nitrate Solution and Ammonium Thiocyanate solution (114)
Experiment 3.20 Fajans Determination of Chloride (116)
Chapter 4 Instrumental Analysis (118)
Experiment 4.1 IRtermination of the Acetylsalicylic Acid in Aspirin Tablet (118)
Experiment 4.2 Determination of Sulfadiazine with Dead-Stop Titration (120)
Experiment 4.3 Evaluation of the Activity Grade for A1uminum Oxide Powder by Column Chromatography (121)
Experiment 4.4 Separating Plant Pigments from Spinach Extract by Column Chromatography (123)
Experiment 4.5 The Identification of Rhizoma Coptidis by Thin Layer Chromatography (125)
Experiment 4.6 Separation of ortho-Nitroaniline and para-Nitroaniline by Thin Layer Chromatography (127)
Experiment 4.7 Identification of Amino acids by Paper Chromatography (128)
Experiment 4.8 Determination of Alcohol in Tincture by Gas Chromatography (130)
Experiment 4.9 Evaluation of the Performance of HPLC Column and IRtermination of Resol ution (132)
Experiment 4.10 Determination of Rutin in Flos Sophorae Immaturus by HPLC (134)
Experiment 4.11 Qualitative and Quantitative analysis of Vitamin~2 Injection (136)
Experiment 4.12 Analysis of Ribof1avin in a Vitamin Tablet by Fluorescence Spectrometry (137)
Experiment 4.13 The Assay of Cuprum in the Cold Medicine (139)
Experiment 4.14 Determination of Benzoic Acid and Benzoic Alcohol by IR Spectrophotometry (141)
Experiment 4.15 Determination of the Structure of Methyl Salicylate by NMR (142)
Experiment 4.16 Quantitative Analysis of Toluene Chlorobenzene and Bromobenzene in a Mixture with Gas Chromatography-Mass Spectrometry Hyphenated Technology (145)
Experiment 4.17 Qualitative and Quantitative Analysis of Baicalin in Radix Scutellariae with Liquid Chromatography-Mass Spectrometry Hyphenated Technology (146)
Chapter 5 Comprehensive and Designable Experiments (150)
Experiment 5.1 Determination of Copper Sulfate in Chalcanthite (Designable Experiment) (150)
Experiment 5.2 Determination of Paeonol in Cortex Moutan (Designable Experiment) (151)
Experiment 5.3 The Separation of Anthraquinone from Radix et Rhizome Rhei by Column Chromatography and the Identification of the Eluted Constituents by Thin Layer Chromatography (Comprehensive Experiment) (151)
Appendix (154)
Appendix 1 Table of Standard Atomic Weights(1995,IUPAC) (154)
Appendix 2 Commonly Used Buffer Solution (155)
Appendix 3 Commonly Used Acid-&se Indicator (155)
Appendix 4 Commonly Used Acid-Base Density，Content and Concentration (156)